In Maulik Patel's lab I have been fortunate to drive the direction of my project. We study mitochondrial DNA as a selfish unit and as a cooperating entity. Mitochondrial DNA exists inside mitochondria and allows for these organelles to be somewhat autonomous. There are many copies of mtDNA per cell and the copy number can vary wildly between cell types. mtDNA replicate from existing mtDNA molecules, but the mechanism of replication regulation is unknown. So, I am working to answer the question: how is mtDNA copy number regulated?

Image of protein purification setup on a chromatography column, with many buffer bottles.

Protein purification setup to generate the antigen against which I made an antibody.

Do experiments.

I am proficient in a variety of molecular, genetic, and biochemical techniques, including:

  • PCR, qPCR, qRT-PCR, ddPCR, transformation, DNA extraction
  • RNAi, genetic crosses
  • Western blotting, CO-IP, ChIP, protein expression and purification, antibody generation